Capillary electrophoresis (CE) is increasingly used in many laboratories to solve the problem of determination of wide physico-chemical properties range substances, including ionic compounds.
The training covers following topics:
- CE fundamentals
- Hands-on training
- Maintenance and troubleshooting
Duration: 4.5 working days / Maximum number of participants: 4
Detailed time schedule:
Day 1 – a.m. – Theoretical session CE
CE Fundamentals:
• Theoretical aspects of CE
• Electrosmotic flow and its modification
• Modes of operation
Day 1 – p.m. – Practical session CE
Experiments and demonstrations related to:
• EOF
• Instrumental aspects
Day 2 – a.m. – Theoretical session CE
CE Fundamentals:
• Instrumental aspects (injection, basic detectors)
• Theoretical background of Capillary Zone Electrophoresis
• Different peak shape
Day 2 – p.m. – Practical session CE
Experiments and demonstrations related to:
• Capillary Zone Electrophoresis
Day 3 – a.m. – Theoretical session CE
CE Fundamentals:
• Theoretical background of Isoelectric Focusing, Isotachophoresis and Gel Electrophoresis
Day 3 – p.m. – Practical session CE
Experiments and demonstrations related to:
• Isoelectric Focusing, Isotachophoresis and Gel Electrophoresis
Day 4 – a.m. – Theoretical session CE
CE Fundamentals:
• SDS/PAGE Electrophoresis
• Qualitative and quantitative analysis
Day 4 – p.m. – Practical session CE
Experiments and demonstrations related to:
• Qualitative and quantitative analysis
Day 5 – a.m. – Practical session CE
Experiments and demonstrations related to:
• Maintenance and troubleshooting